Endomorphin-1&2
(Cat #:MV1005)
| Product Description |
Rabbit polyclonal antibody to synthetic Endomorphin-1 (cross-reacts with Endomorphin-2) |
 Immunohistochemical staining of Endomorphin-1&2 in rat spinal cord dorsal horn. Brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to Endomorphin-1&2 at 5 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit FITC secondary antibodies at 1:100. |
| Formulation |
Lyophilized from 10 mM PBS. |
| Purification |
Whole IgG |
| Host Species |
Rabbit |
| Unit Size: |
50 µg |
| Immunogen |
Endomorphin-1 conjugated to BSA. |
| Sequence: |
YPWF |
| Antigen Location (aa): |
1-4 |
| Alternative Names |
N/A |
| Accession Number: |
Precursor protein is not identified. |
| Accession Name: |
Precursor protein is not identified. |
| Accession URL: |
Precursor protein is not identified. |
| Function: |
Endomorphins 1 and 2 are endogenous opioid peptides which have the highest affinity for the mu-opioid receptors. Located in various parts of the brain and interact with mu-opioid receptors and produces analgesia. |
| Applications: |
Recommended for Immunohistochemistry (IHC) and Cytochemistry (ICC). |
| Working Dilution for Immunofluorescence (ICC): |
5 – 15 µg/mL |
| Working Dilution for Immunohistochemistry (IHC): |
5 – 10 µg/mL |
| IHC Positive control: |
Brain (spinal cord, PAG, DRG). |
| Specificity: |
Confirmed by RIA against the synthetic peptide. |
| Cross-reactivity: |
Human; mouse; rat. Endomorphin-1 a highly conserved molecule, so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Endomorphin-2 <5%; Met-enkephalin <0.03%; with Leu-enkephalin 0.01%; with beta-endorphin<0.1%. |
| Reconstitution: |
Reconstitute in 0.05 mL of PBS (pH 7.4) to achieve an antibody concentration of 1000 µg/mL. Centrifuge to remove any insoluble material. |
| Storage / Stability: |
At least 12 months after purchase at 2 - 4°C. After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles. |
References:
- Zadina JE, et al. A potent and selective endogenous agonist for the mu-opiate receptor. Nature. 1997; 386(6624):499-502. PMID: Zadina JE, Hackler L, Ge LJ, Kastin AJ. Nature. 1997 Apr 3;386(6624):499-502. PMID: 9087409.
- Hackler L, et al. Isolation of relatively large amounts of endomorphin-1 and
endomorphin-2 from human brain cortex. Peptides. 1997; 18(10):1635-9. PMID: 9437727.
- Schreff M, et al. Immunofluorescent identification of endomorphin-2-containing nerve fibers and terminals in the rat brain and spinal cord. Neuroreport. 1998; 20;9(6):1031-4. PMID: 9601662.
- Zadina JE.Isolation and distribution of endomorphins in the central nervous system. Jpn J Pharmacol. 2002 Jul;89(3):203-8. Review. PMID: 12184722.
- Hui R, Chen T, Li YQ. Neuroscience. 2006;138(1):171-81. PMID: 16360287.
- Greenwell TN, Martin-Schild S, Inglis FM, Zadina JE. J Comp Neurol. 2007 Jul 10;503(2):319-33. PMID: 17492626.
- Lv BC, Ji GL, Huo FQ, Chen T, Li H, Li YQ. J Chem Neuroanat. 2010 May;39(3):166-74. PMID: 19931378.
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